Sequencing Applications

NGS-based DNA sequencing generates millions of short reads in parallel to decode genetic information at scale. By aligning sequencing reads to a reference genome or assembling them de novo, it enables accurate identification of genetic variation across targeted regions, exomes, and whole genomes.

NGS-based RNA sequencing (RNA-seq) converts RNA into cDNA fragments through reverse transcription, followed by high-throughput short-read sequencing. Without relying on predefined microarray probes, RNA-seq provides unbiased digital quantification, enabling accurate measurement of gene expression abundance across an ultra-wide dynamic range.

Epigenetics focuses on heritable gene expression changes that occur without altering the underlying DNA sequence. By utilizing chemical conversion (such as bisulfite or enzymatic tools), antibody-based enrichment, or transposase cleavage, NGS converts epigenetic modifications into short-read data that can be precisely quantified and mapped across the genome.